Latest Article






Title : Ivf versus spontan singleton pregnancies fetal and maternal outcomes in a tertiary care hospital


Background: We aimed to compare perinatal outcomes of intracytoplasmicsperm injection (ICSI) versus naturally (spontaneously) concieved age and parity matched group of singleton pregnants.

Methods: Two hundred and sixteen singleton pregnancies aged between 18-45 years old were included in this study.  Among all study group, 106 cases were ICSI pregnancies (study group) and 110 cases were spontaneously concieved singleton pregnancies (control group).  Pregnancy outcome parameters were: the incidence of chronic hypertension, preeclampsia, gestational hypertension, placenta previa, placental abruption, preterm birth, intrahepatic cholestasis of pregnancy, gestational diabetes, preterm premature rupture of membranes, caesarean delivery.

Results: There were no differences in terms of maternal age, BMI, gravidity, parity, gestational weeks at birth and birth weight between ICSI and spontaneously concieved pregnancy groups. Placental abruption, gestational diabetes and cesarean section rates were significantly higher in ICSI pregnancies than spontaneously conceived pregnancies (4.7% vs 0%, 21.7% vs 11.8% and 82.1% vs 68.2%, respectively). There wereno statistically significant differences in terms of chronic hypertension, preeclampsia, gestational hypertension, preterm labor, placenta previa, intrahepatic cholestasis of pregnancy, preterm and term premature rupture of the membranes.

Conclusion: ICSI pregnancies have higher adverse perinatal outcomes than spontaneously conceived singleton pregnancies which were matched with age and parity. That’s why ICSI pregnancies should be given a detailed counseling about the adverse perinatal outcomes and should be followed up more carefully through the pregnancy.


First invitro fertilization (IVF) baby, Louise Brown was born in 1 978 in the United Kingdom[ 1 ]. After than, first ICSI procedure was performed successfully in 199 2 . Especially ıt was a great chance for male infertility who hadsevere sperm defects [2]. So this method was hopeful for the couples who were unsuccesful with conventional IVF methods.

Although there are many pregnancies born with healthy outcomes in ART cycles, there are also many reports on increased risks of maternal, fetal and neonatal outcomes (such as preeclampsia, plasenta previa, gestational diabetes, preterm labor, premature rupture of membranes, ablatio placenta, low birth weight etc.) in IVF pregnancies compared to the naturally conceived pregnancies [ 3 , 4 , 5 , 6 ]. Although, the majority of the adverse outcomes related to the multiple pregnancies[ 7 ], singleton pregnancies with IVF cycles have also increased

obstetrical and neonatal risks compared to the naturally concieved singleton pregnancies. Micromanuplations in oocyctes, sperms and zygotes, medications and culture systems used maybe the cause of abnormal implantation in endometrium and cause these adverse outcomes.

We aimed to compare the obstetric and neonatal outcome parameters of singleton ICSI pregnancies with naturally concieved singleton pregnancies in ART department of our tertiary obstetric clinic.


This retrospective study was conducted between February 2012 and April 2013 in Obstetric Department of Zeynep Kamil Women and Children Diseases Training and Research Hospital in Istanbul. The pregnancies leading to a live birth (>28 weeks gestation or >1200 g birth weight) were included in this retrospective cohort study. A total of 216 singleton pregnant women ages between 18-45 years old were included in this study, there were 106 cases who conceived by ICSI(study group) while 110 cases conceived spontaneously(control group). Groups were matched in terms of age and parity.

Maternal demographic parameters such as age, body mass index (BMI), gravidity, parity, gestational age at birth; obstetric complications such as preeclampsia(blood pressure >140-90 mm-Hg and proteinuria>300mg/day after 20 weeks gestation), gestational hypertension (blood pressure >140-90 mmHg without proteinuria onset during gestation), chronic hypertension (blood pressure>140-90mmHg before gestation), gestational diabetes (diagnosed after 100 gr glucose loading test according to Carpenter-Couston criteria), placental previa and placental abruption, hepatic cholestasis, preterm membrane rupture(rupture of membranes after 37 weeks of gestation before contractions), premature preterm membrane rupture(rupture of membranes before 37 weeks gestation), mode of delivery, birth weight and fetal complications such as neonatal intensive care unit (NICU) stay, respiratory distress syndrome and hospital stay more than 10 days were all recorded.

Data were obtained from maternal and neonatal delivery and hospitalization records. Ethics committee approval was obtained from Zeynep Kamil Women and Children Diseases Training and Research Hospital Ethics Committee. (Date and number 23rd of may 2014- 76)

Statistical analyses

Statistical analyses were performed using SPSS software program. Demographic characteristicswere presented as mean ±standard deviations or frequenciesor percentages. Comparisons between ICSI and spontaneous singleton pregnancies variables for maternal and fetal results t-tests (Wilcoxon-tests) or chi2-tests (Fisher’s exact tests) were used. P-values <0.05 were considered statistically significant.


The maternal and fetal demographic characteristics in both groups were shown in Table 1.

Table 1 Demographic characteristics.

ICSI pregnancies mean (standart deviation) Spontaneous pregnancies mean (standart deviation) P value
Age (year) 32.37±4.80 32.36±4.88 p>0.05
BMI (kg/m2) 26.23±4.77 30.02±2.85 p>0.05
Gravida 1.29±0.57 1.25±0.49 p>0.05
Parity 0.17±0.44 0.15±0.40 p>0.05
Gestational weeks at birth (days) 258.5±23.07 259.9±23.46 p>0.05
Birth weight (gram) 2863.86±770.42 2797.48±770.19 p>0.05

There were no differences between ICSI and spontaneously conceived pregnancies in terms of maternal age, BMI, gravidity, parity, gestational weeks at birth and birth weight.

Obstetric outcomes were shown in Table 2.

Table 2: Obstetric outcomes.

ICSI pregnancies N=number (%) Spontaneous pregnancy N=number (%) P value
Chronic hypertension 3 (2.8) 2 (1.8) P>0.05
Preeclampsia 14 (13.2) 18 (16.6) p>0.05
Gestational hypertension 10 (9.5) 4 (3.6) p>0.05
Preterm labor 19 (18.1) 11 (10.0) p>0.05
Ablatio placenta 5 (4.7) 0 ( 0 ) P<0.05
Placenta previa 5 (4.7) 1(0.9) p>0.05
Intrahepatic cholestasis of pregnancy 4 (3.8) 3 (2.7) p>0.05
Gestational diabetes 22 (21.7) 13 (11.8) P<0.05
Preterm premature rupture of membranes 7 (6.6) 4 (3.6) p>0.05
Preterm rupture of membranes 7 (6.6) 9 (8.2) p>0.05
Cesarean section 87 (82.1) 75 (68.2) P<0.05

7% vs 0%; 217% vs 118% and 821% vs 682%) There were no statistically significant differencesin the rates chronic hypertension, preeclampsia, gestational hypertension, preterm labor, placenta previa, intrahepatic cholestasis of pregnancy, preterm and term premature rupture of the membranes

Neonatal outcomes were shown in table 3.

ICSI pregnancies N=number (%) Spontaneous pregnancies N=number (%) P value
Respiratuar distress syndrome 19 (18.3) 20 (18.3) p>0.05
NICU admission 28 (26.4) 39 (35.5) p>0.05
Hospitalisation longer than 10 days 2 (1.9) 7 (6.4) p>0.05

Neonatal outcomes

There were no statistically significant differences in terms of the rates of neonatal respiratory distress syndrome, NICU admission and neonatal hospitalization days longer than 10 days between the two groups.


There are many reports in the literature that IVF pregnancies have higher adverse pregnancy outcomes, but most of them did not studied only singleton pregnancies and none of them reported maternal age adjusted complication rates [ 8 , 9 ]. We know that the incidence of adverse obstetric outcomes such as preeclampsia, gestational diabetes, placental abruption, placenta previa and abnormal labor have been observed with a higher ratein women with advanced ages [ 10 , 11 ] .That’s why we have chosen maternal age and parity matched singleton pregnancies in control group.

We found higher rates of ablatio placenta, gestational diabetes and cesarean section rates in ICSI pregnancies than spontaneously conceived pregnancies.

Suzuki et al. [ 12 ] concluded that in elderly pregnant patients (age>35 years) cesarean rates were higher in IVF pregnancies than spontaneously conceived pregnancies but they found no statistical difference between other obstetric outcomes between the groups. Also Suzuki et al., stated that placental abruption in over 35 years old patients conceived by IVF was observed with four fold increased rate compared to spontaneously conceived 20-29 years old patients. However, the differenceswere not statistically significant in both elderly (over 35 years old patients) IVF and spontaneously conceived groups. In our study,we selected matched age control groups, and found higher placental abruption rates in ICSI group of patients than spontaneously conceived patients. Suzuki et al. [ 13 ] also studied obstetric outcomes of IVF patients over 35 years old and below 35 years old IVF patients. They found no difference in means of placental abnormality, premature delivery or neonatal asphyxia between the two groups. Hence they concluded that obstetric complications in pregnancies conceived by IVF are attributed to mechanisms other than those that depend on advanced maternal age.

Dayan et al. [ 14 ] compared IVF and ICSI singleton pregnancies with spontaneously conceived pregnancies in a population based study, and concluded that the rate of chronic hypertension and gestational diabetes rates were statistically higher in ART pregnancies than spontaneously conceived pregnancies. They also concluded in the same study that, increased placental complications were accompanied with hypertension. In our study we found similar the rates of chronic hypertension, gestational hypertension and preeclampsia between ICSIand spontaneouslyconceived pregnancies. But we also found statistically significantly higher rates of placental abruption. So In contrast to Dayan hypothesis, we believe, ART was an independent risk factor for placental complications.

Egbe et al. [ 15 ] showed higher cesarean rates in IVF pregnancies than the naturally conceived ones (4.1 times more), which supports our results. They included 102 cases (51 IVF and 51 control cases), in their study, both singleton and multiple pregnancies were included. They did not evaluate placental abruption, gestational diabetes and they did not findany significant differencebetween the groups in terms of birth weight, gestational age at delivery and Apgar scores of neonates. As the maternal and fetal complications were high in multiple pregnancies, we only included singleton pregnancies.

Cesarean section rates were so high in our study in both groups. Because our hospital is tertiary referral hospital and majority of the cases arehigh risk pregnancies . In our study, history of previous caesarean rates were also disregarded. Increased cesarean rates in study group weremainly due to high anxiety levels of both patients and obstetricians about adverse outcomes of fetus and the mother.Also increased gestational diabetes rates which resulted in increased cesarean rates.

Nouri et al.[ 16 ] studied obstetric and perinatal outcomes in IVF versus ICSI-conceived pregnancies and they concluded that the birth weight and 1 and 10 minute Apgar scores were lower in ICSI group than IVF group and ICSI-conceived children were more often hospitalized at a neonatal intensive care unit than IVF children.All our cases were ICSI patients so if we had used IVF procedure instead of ICSI, the adverse outcomes would have decreased or disappeared.

Dhont et al.[ 17 ] studied perinatal outcome of IVF and ICSI pregnancies in case control study. They found no differences in perinatal outcomes (birth weigth, perinatal mortality, mean hospital stay, NICU admission) in ART singleton pregnancies than age, parity matched singleton controls. But ART cases were either IVF or ICSI, they did not compare each group with control groups.

Jackson et al [ 18 ], reported a meta-analysis about perinatal outcomes in singletons following in vitro fertilization. Fifteen studies comprising 12,283 IVF and 1.9 million spontaneously conceived singletons were included in that meta-analysis. They found that compared with spontaneous conceptions, IVF singleton pregnancies were associated with significantly higher odds ratios: perinatal mortality (odds ratio [OR] 2.2; 95% confidence interval [CI] 1.6, 3.0), preterm delivery (OR 2.0; 95% CI 1.7, 2.2), low birth weight (OR 1.8; 95% CI 1.4, 2.2), very low birth weight (OR 2.7; 95% CI 2.3, 3.1), and small for gestational age (OR 1.6; 95% CI 1.3, 2.0). Early preterm delivery, spontaneous preterm delivery, placenta previa, gestational diabetes, preeclampsia, and neonatal intensive care admission were also significantly more prevalent in the IVF group. So, they concluded that IVF patients should be informed of the increased risk for adverse perinatal outcomes.

Allen et al [ 19 ], reported that singleton ART pregnancies have an increased risk of preterm delivery and low birth weight at term. Non-lethal congenital malformation rates are not increased following ART. Placenta previa was increased following ART. We found no difference in means of placenta previa, preterm delivery and low birth weight rates in our study.

Tsutsumi et al.[ 20 ] studied singleton pregnancy outcomes after assisted and non-assisted reproductive technology in infertile patients. They studied Pregnancy outcome parameters such as pregnancy induced hypertension, placenta previa, placental abruption, cesarean delivery, preterm birth, very preterm birth, stillbirth, low birth weight and very low birth weight. They concluded that only ART procedure may be a risk factor for the development of placenta previa. Other abnormal perinatal outcomes that had been previously attributed to ART, may be due to the baseline characteristics of infertile patients.

In a systematic review [ 21 ], Shevell et al. [ 3 ] found that increased maternal complications such as 6 fold high increased placenta previa, 1.6 fold high gestational hypertension, 0.5 fold higher gestational diabetes, 2.4 fold high placental abruption, 2 fold higher caesarean delivery rates. Kallen et al. [ 22 ] and Romundstad et al. [ 23 ] found increased placenta previa rates in IVF cycles than naturally conceived pregnancies, 4 fold and 6 fold higher respectively. Allen et al. [ 24 ] also emphasized increased maternal complications in IVF pregnancies, 2 fold higher in gestational hypertension, gestational diabetes and caesarean deliveries than naturally conceived singleton pregnancies. We only found higher rates of gestational diabetes, placental abruption and caesarean rates in our study. We did not findany difference in terms of placenta previa rates.

As in our study,Silberstein et al. [ 25 ] (in a large population based study, IVF; n=1296 and singleton pregnancies conceived spontaneously, n=172 288) pointed out increased gestational diabetes risk in IVF pregnancies than conceived spontaneously (OR=1.77, 95% CI:1.52–2.07, p<0.001).

Limitations : As we included the patients from delivery (maternity) hall in our hospital we couldn’t compare the congenital anomaly rates including aneuploidies and epigenetic or imprinting disorders andmajor birth defectsin both groups andthe sample size were small for both the study and control groups. Long-term outcomes such as neuro-developmental or neuro-behavioral development of the child in ART pregnancies were not studied.


As the pregnancies achieved by ICSI have higher adverse perinatal outcomes; closer follow-up should be done and careful counseling should be offeredto this pregnant population. Detailed studies, done with fresh or frozen embrios, IVF or ICSI procedures and embrio transfer at 3rd or 5th day of embrio may have different perinatal outcomes.

Conflict of interest

The authors report no conflict of interest

Founding source

The authors certify that they have no funding source for the study. The authors declare no relation with funding sources or sponsorships.


We warmly thank Dr.Tayfun Kutlu for his expert collaboration in statistical analysis.


  1. BIRTH AFTER THE REIMPLANTATION OF A HUMAN EMBRYO Steptoe PC, Edwards RG. The Lancet.1978-aug. Google Scholar
  2. Does Intracytoplasmic Sperm Injection Pose an Increased Risk of Genitourinary Congenital Malformations in Offspring Compared to In Vitro Fertilization? A Systematic Review and Meta-Analysis Massaro PeterA, MacLellan DawnLee, Anderson PeterA, Romao RodrigoLP. The Journal of Urology.2015-may;:1837-1842. Google Scholar
  3. Assisted Reproductive Technology and Pregnancy Outcome Shevell Tracy, Malone FergalD, Vidaver John, Porter TFlint, Luthy DavidA, Comstock ChristineH, Hankins GaryD, Eddleman Keith, Dolan Siobhan, Dugoff Lorraine, Craigo Sabrina, Timor IlanE, Carr StephenR, Wolfe HonorM, Bianchi DianaW, D’Alton MaryE. Obstetrics & Gynecology.2005-nov;:1039-1045. Google Scholar
  4. Smaller fetal size in singletons after infertility therapies: the influence of technology and the underlying infertility Cooper AmberR, O’Neill KathleenE, Allsworth JeniferE, Jungheim EmilyS, Odibo AnthonyO, Gray DianaL, Ratts ValerieS, Moley KelleH, Odem RandallR. Fertility and Sterility.2011-nov;:1100-1106. Google Scholar
  5. Neonatal outcome of IVF singletons versus naturally conceived in women aged 35 years and over Tomic Vlatka, Tomic Jozo. Archives of Gynecology and Obstetrics.2011-mar;:1411-1416. Google Scholar
  6. Preterm birth and low birth weight among in vitro fertilization singletons: A systematic review and meta-analyses McDonald SarahD, Han Zhen, Mulla Sohail, Murphy KellieE, Beyene Joseph, Ohlsson Arne. European Journal of Obstetrics & Gynecology and Reproductive Biology.2009-oct;:138-148. Google Scholar
  7. Selected neonatal outcomes in dizygotic twins after IVF versus non-IVF pregnancies Källén B, FinnstrĂśm O, Lindam A, Nilsson E, Nygren K.-G., Olausson POtterblad. BJOG: An International Journal of Obstetrics & Gynaecology.2010-feb;:676-682. Google Scholar
  8. Infertility, Assisted Reproductive Technology, and Adverse Pregnancy Outcomes Reddy UmaM, Wapner RonaldJ, Rebar RobertW, Tasca RichardJ. Obstetrics & Gynecology.2007-apr;:967-977. Google Scholar
  9. Perinatal Outcomes in Singletons Following In Vitro Fertilization: A Meta-Analysis Jackson RebeccaA, Gibson KimberlyA, Wu YvonneW, Croughan MaryS. Obstetrics & Gynecology.2004-mar;:551-563. Google Scholar
  10. Maternal age and pregnancy outcome Naeye R: Maternal age, obstetric complications, and the outcome of pregnancy. Obstet Gynecol 61:210, 1983 Journal of Nurse-Midwifery.1983-may. Google Scholar
  11. Health risks for early (?19) and late (?35) childbearing Ozalp Sinan, Tanir HMete, Sener Turgay, Yazan Sibel, Keskin AliE. Archives of Gynecology and Obstetrics.2003-aug;:172-174. Google Scholar
  12. Obstetric outcomes of elderly primiparous singleton pregnancies conceived byin vitrofertilization compared with those conceived spontaneously SUZUKI SHUNJI, MIYAKE HIDEHIKO. Reproductive Medicine and Biology.2007-nov;:219-222. Google Scholar
  13. Obstetric outcomes in nulliparous women aged 35 and over with singleton pregnancies conceived by in vitro fertilization Suzuki Shunji, Miyake Hidehiko. Archives of Gynecology and Obstetrics.2007-sep;:225-227. Google Scholar
  14. Effect of chronic hypertension on assisted pregnancy outcomes: a population-based study in Ontario, Canada Dayan Natalie, Lanes Andrea, Walker MarkC, Spitzer KarenA, Laskin CarlA. Fertility and Sterility.2016-apr;:1003-1009. Google Scholar
  15. In-vitro fertilization and spontaneous pregnancies: matching outcomes in Douala, Cameroon Egbe ThomasObinchemti, Sandjon Guy, Ourtchingh Clovis, Simo André, Priso EugeneBelley, Benifla Jean-Louis. Fertility Research and Practice.2016-jan. Google Scholar
  16. Obstetric and perinatal outcomes in IVF versus ICSI-conceived pregnancies at a tertiary care center - a pilot study Nouri Kazem, Ott Johannes, Stoegbauer Lucia, Pietrowski Detlef, Frantal Sophie, Walch Katharina. Reproductive Biology and Endocrinology.2013. Google Scholar
  17. Dhont M, Neubourg FDe, Elst JVander, Sutter PDe. Journal of Assisted Reproduction and Genetics.1997;:575-580. Google Scholar
  18. Perinatal Outcomes in Singletons Following In Vitro Fertilization: A Meta-Analysis Jackson RebeccaA, Gibson KimberlyA, Wu YvonneW, Croughan MaryS. Obstetrics & Gynecology.2004-mar;:551-563. Google Scholar
  19. Pregnancy and perinatal outcomes after assisted reproduction: a comparative study Allen C, Bowdin S, Harrison RF, Sutcliffe AG, Brueton L, Kirby G, Kirkman-Brown J, Barrett C, Reardon W, Maher E. Irish Journal of Medical Science.2008-jun;:233-241. Google Scholar
  20. Singleton pregnancy outcomes after assisted and non-assisted reproductive technology in infertile patients Tsutsumi Ryo, Fujimoto Akihisa, Osuga Yutaka, Ooi Nagisa, Takemura Yuri, Koizumi Minako, Yano Tetsu, Taketani Yuji. Reproductive Medicine and Biology.2012-mar;:149-153. Google Scholar
  21. Reproductive outcomes after in-vitro fertilization Mukhopadhaya Neelanjana, Arulkumaran Sabaratnam. Current Opinion in Obstetrics and Gynecology.2007-apr;:113-119. Google Scholar
  22. In vitro fertilisation in Sweden: obstetric characteristics, maternal morbidity and mortality Kallen Bengt, Finnstrom Orvar, Nygren KG, Olausson PetraOtterblad, Wennerholm Ulla-Britt. BJOG: An International Journal of Obstetrics and Gynaecology.2005-nov;:1529-1535. Google Scholar
  23. Increased risk of placenta previa in pregnancies following IVF/ICSI$\mathsemicolon$ a comparison of ART and non-ART pregnancies in the same mother Romundstad LB. Human Reproduction.2006-jun;:2353-2358. Google Scholar
  24. Pregnancy Outcomes After Assisted Reproductive Technology Allen VictoriaM, Wilson RDouglas, Cheung Anthony, Blight Claire, Désilets ValerieA, Gagnon Alain, Langlois SylvieF, Summers Anne, Wyatt Philip, Claman Paul, Goodrow Gwen, Graves Gillian, Min Jason. Journal of Obstetrics and Gynaecology Canada.2006-mar;:220-233. Google Scholar
  25. Perinatal outcome of pregnancies followingin vitrofertilization and ovulation induction Silberstein Tali, Levy Amalia, Harlev Avi, Saphier Oshra, Sheiner Eyal. The Journal of Maternal-Fetal & Neonatal Medicine.2013-nov;:1316-1319. Google Scholar


Download data is not yet available.

We Recommend

Author's Affiliation
Article Details

Issue: Vol 5 No 3 (2018)
Page No.: 3583-3587
Section: Articles

 How to Cite
Çakar, E., Yavuz, M. M., Kutlu, T., Taşan, H. A., Cogendez, E., Nacak, G. B., Özkaya, E., & Eser, S. K. (2018). Ivf versus spontan singleton pregnancies fetal and maternal outcomes in a tertiary care hospital. International Journal Of Medical Science And Clinical Invention, 5(3), 3583-3587.

 Cited by

Article level Metrics by Paperbuzz/Impactstory:

 Statistics at Article Level
HTML = 28 times
PDF   = 11 times
XML   = 0 times
Total   = 11 times