Form deprivation (FD) is an effective approach to induce animal models of myopia. It disrupts the normal growth process, induces rapid axial elongation, and results in myopia in many species. In this investigation, three weeks old rat was deprived of form vision for 4 weeks and then compared to the fellow, non-deprived eye by histopathology of scleral thickness and fibroblast.
Ten of male three-weeks-old wistar rat weighing 200–300 g were obtained from the Airlangga University Laboratory Animal Center (Surabaya, Indonesia). All animals were examined clinically for confirming the corneal transparency of each eye and no injuries or infections of the eyes. Refractive errors were induced monocularly by covering right eye with adhesive tape in one group for 4 weeks which was named the FDM group. After enucleated under deep anesthesia histopathology of scleral thickness and fibroblast
There were no differences in baseline axial length variance among the groups (F = 1.006, P = .413). Statistical analysis using t-test, scleral thickness in the treatment group was reduced compared with the control group but the difference was not statistically significant (p =0.443; p > 0.05). Scleral fibroblast in the treatment group was reduced compared with the control group but the difference was not statistically significant (p= 0.990; p > 0.05). Logistic regression analysis showed that association of form deprivation myopia, scleral thickness and scleral fibroblat was not significant (p=0.997)
The scleral thickness and scleral fibroblast at conclusion of our study were reduced but not significant than in the control group. These results not validate the FDM model, further study with more samples and longer FDM periods is necessary.
Dhany, MD, R. K. (2018). Comparison of Scleral Thickness and Fibroblast in Form Deprivation Animal Model (Experimental Study of Wistar Rat). International Journal of Medical Science and Clinical invention, 5(5), 3803-3806. https://doi.org/10.18535/ijmsci/v5i5.09