Abstract

Plants play an important role as a source of many anticancer drugs, thus screening of these plants in any region in our world may give a new valuable drug against cancer. The mechanism of anti-proliferative activity of new candidate plant extracts (Glycoside extract Fraction I from leaves of Convolvulus arvensis ) as anticancer drug against RD tumor cell line after 72hrs treatment was indicated previously by the same investigators need to explanation. Glycoside was separated and fractionated by Thin Layer Chromatography (TLC) using silica gel. This study was designed to describe the activity of glycoside extract Fraction I(FI) from leaves of Convolvulus arvensis against the proliferation of RD cells and attempt to get an integrated view and better understanding of its modes of action . The result of present study showed that the Rate of flow (Rf) of glycoside fraction I was 0.8,whereas the melting point was 160 oC. This study demonstrated that the glycoside extract Fraction I from leaves of Convolvulus arvensis caused apoptosis. Since the light and fluorescent microscope observation showed morphological characteristic of apoptosis including cell volume shrinking, chromatin condensation and nuclear fragmentation after the treatment of RD tumor cell line with different concentrations (1250, 2500, 5000, and 10000, μg/ml) of glycoside at 72hrs. Beside that the apoptotic effect was confirmed by DNA fragmentation into low molecular weight fragments 180-200 pb as well as fragments with high molecular weight by using of agarose gel electrophoresis when treated with different concentrations.