Uropathogenic E. coli (UPEC) is responsible for approximately 90% of urinary tract infections 1 . Abuse and improper prescribing policy of antibiotics causes remarkable increase of antibiotic resistance pattern among the E. coli isolates from UTI2 . These types of resistance are associated with genetic mutation and intra or inter species transfer of resistance gene through plasmid3 . Therefore regional studies on pattern of antibiotic sensitivity are very much necessary to overcome this problem. Considering the majority of UTI cases caused by E. coli and increasing use of antibiotics followed by growing resistance in bacteria and emerging MDR strains, the present study was conducted to identify the UPEC and also investigate the drug resistance pattern of those E. coli strains collected from clinical sample.