Articles | Open Access
Vol. 5 No. 3 (2018)
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Page No.: 3641-3647 |
https://doi.org/10.18535/ijmsci/v5i3.16
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria ,,Département de Biologie, faculté des Sciences de la Nature et de la Vie, Mustapha Stambouli University of Mascara, Mascara, Algeria
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Abbassia Benchaachoua
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria
Département de Biologie, faculté des Sciences de la Nature et de la Vie, Mustapha Stambouli University of Mascara, Mascara, Algeria
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria ,, Department of Biology, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria
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Hassiba Mahdjoub Bessam
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria
Department of Biology, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria ,,Département de Biologie, faculté des Sciences de la Nature et de la Vie, Mustapha Stambouli University of Mascara, Mascara, Algeria
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Imene Saidi
Laboratory Eco Development of Areas, Faculty of Natural Sciences & Life, Djillali Liabes University of Sidi Bel-Abbes, Sidi Bel-Abbes, Algeria
Département de Biologie, faculté des Sciences de la Nature et de la Vie, Mustapha Stambouli University of Mascara, Mascara, Algeria
Abstract
Objective: The objectives of this study were essentially to evaluate the best technique for extracting phenolic compounds from Silybum marianum leaves.
Subjects and Methods: Two extraction methods (maceration and decoction), using four extraction solvents. The efficiency of the extraction methods was estimated by quantifying the total polyphenol contents by the Folin -Ciocalteu method and by determining the antioxidant power of the various extracts by the ability to trap the free radical DPPH °, and the influence of these parameters on the antioxidant activity.
Results: The results showed that the extracts of the leaves by maceration have contained the highest percentage yield (24.68%); the highest content in the aqueous -methanol polyphenols extract (18.75 ± 0.55 mg GAE / g DW ) against the decoction is more effective for the extraction of flavonoids and condensed tannins. The highest content of flavonoids had been recorded for the aqueous- ethanolic extract (5.08 ± 0.57 CE mg / g DW). The water had the highest content of condensed tannin (03.86 ± 0.22 TAE / g DW). On the other hand, the aqueous -ethanolic and aqueous -methanol extract of the leaves obtained by maceration have the best antioxidant capacity (73.84 ± 5.90 EAG / g DW and 73.64 ± 5.93 mg EAG / g DW respectively) and the aqueous extract of the leaves by decoction had a better antiradical power (48.64 μg / ml).
Conclusion: The extraction of phenolic compounds from Silybum marianum leaves was strongly influenced by the type of solvent and the extraction method. In addition, it is very difficult to choose an appropriate extraction solvent for all plant samples.
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Copyright © 2018
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Abbassia Benchaachoua,
Hassiba Mahdjoub Bessam,
Imene Saidi, this is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.