In Vitro Anti-Microbial and Anti-Fungal Activity of Successive Extract of G. Asiatica Linn. Leaf.

Abstract

Background / Aims: Enterobacter species are associated with bloodstream, soft tissue, respiratory and urinary tract infections in humans. E.cloacae, E.aerogenes and E.asburiae are the most frequently isolated Enterobacter species. It was aimed to investigate antimicrobial resistance among Enterobacter spp. strains isolated from clinical specimen.

Antibacterial activity of alcoholic extract of G. asiatica Linn leaves were comparable to standard (Streptomycin) and aqueous extract was less active than standard against Gm +ve (B.  subtilis)  organism. Antibacterial activity of aqueous extract was less than the standard (Ampicillin) against Gm –ve (E. coli) organism. Antifungal activity of aqueous extract was comparable to standard (Fluconazole) for S. cerevisae.

Materials and Methods: This retrospective study included Enterobacter spp. strains isolated from clinical specimen sent from Dicle University Hospital clinics from 2015 to 2017. The isolates to be considered as infection agents were identified by Maldi Biotyper 3 (Bruker Daltonics, USA). The antimicrobial susceptibility test (ADT) was carried out by BD Phoenix 100 (Becton Dickinson, USA) system and evaluated according to EUCAST v.8.0 criteria.

Results: A total of 349 (115 in 2015, 112 in 2016, and 122 in 2017) Enterobacter spp. strain was isolated in three-years period. The most frequently identified species were E.cloacae (240 strains, 68.7%) and  E. aerogenes (53 strains, 15.1%). The lowest resistance was determined against amikacin, meropenem and ciprofloxacin. Conclusion: Amikacin and meropenem were the most effective antibiotics for E. aerogenes and E. cloacae strains. Resistance rates of other Enterobacter strains other than E. cloacae and E. aerogenes.varied according to years and species.