PLGA Based Nanoencapsulation For Controlled Release Of Doxorubicin For Cancer Therapy

Abstract

Nanoparticle based drug delivery has been evolved to enhance the drug efficacy in recent times. Nanoparticles are used for drug delivery should be less toxic for normal cells and more effective drug delivery for tumor cells. In this study, the doxorubicin is encapsulated with Poly Lactic and Glycolic acid (PLGA) by desolvation technique. The doxorubicin loaded PLGA nanoparticles are prepared by desolvation technique. Ethyl alcohol was added intermittently into PLGA solution. Following the desolvation of the doxorubicin, the resulting nanoparticles were stabilized by the addition of glutaraldehyde. The morphology and characterization of nanoparticles were done by using Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR). The drug release study was carried by in vitro setup. It was noted that 82% of drug has been released at the end of 72 hours. In addition, animal cell line study was done for anti-cancer efficiency. PLGA polymeric particles loaded with doxorubicin act as a promising vehicle for targeting tumor cells by killing them at drug concentration of as low as 50µg/ml