Abstract
Anti human globulin test (AHG) comprising of both Direct Coombs’ Test (DAT) and Indirect Coombs’ Test (IAT) can be used to detect RBC sensitized with Ig G auto and alloantibodies, complement occurring in vivo and vitro. The present study was conducted in 204 cases on which 360 tests comprising of both DAT and IAT were carried out. Out of 29 positive Coombs’ cases, 11 were positive for DAT, 16 were positive for IAT and 02 had both DAT and IAT positivity. Maximum cases DAT and IAT positive cases were autoimmune hemolytic anemia (AIHA) and Rh incompatibility respectably. In our set up 5.6 % cases were DAT positive and 9.3 % cases were IAT positive. Any immune-hematological work up is incomplete without the intervention by anti-globulin tests which should be mandatory for the better management of patients.