Morphometrical and histological evaluation of the effect of vitamins B1, B6 and B12 on rats sciatic nerve after crush injury

Abstract

Objectives: To evaluate the effect of vitamins (B1, B6, and B12) on nerve regeneration and recovery after crush injury to sciatic nerve in experimental rats Study Design: Prospective observational study Setting: Department of anatomy, College of medicine,Baghdad University Subjects and intervention: Total 60 Rats ,with ageing(8-12) weeks old of both sex, during 45 days period were included in this study Study period: 15 October 2016 to 30 November 2016 Results: on comparision among different groups ( control and experimental groups ) . Best improvement in group of rats on B12(group D) than other groups on B1(groupB) or B6(group C). B12 group treated with ( 1mg / kg / day ) significantly increased in the MTC when compared to the control , B6 and B1 groups on days 15, 30 and 45 of the study at P≤0.05. Histological analysis by Van Giesons stain,A- all groups control and experimental (B1,B6,B12,A) show degeneration in different areas but less in group B12. B- sciatic nerve fascicles with Schwann cell and fibroblast better organized in group B12 Conclusion: vitamin B12, among different kind of vitamins B, considered as a best vital medication for regeneration and remyelination of damage nerve fibers.