Aim – To evaluate the role of pan-endoscopy in the diagnosis of abdominal tuberculosis in HIV negative adult. Materials and Methods – This is a Prospective observational study carried out in a Tertiary care hospital over a period of one year. All adults’ patients with suspected abdominal tuberculosis were evaluated with detailed clinical examination, relevant laboratory tests, trans-abdominal ultrasonography and ascitic fluid analysis. Upper gastrointestinal endoscopy and colonoscopy was done wherever feasible and biopsy samples were collected for crush smears for Z-N staining and histopathological examination. Patients who are found positive in HIV screening were excluded from the study. Result – A total of 50 patients (26 females and 24 males) were studied over a period of 1 year. The mean age of patients was 28.7 (±9.31) years. The presenting features in isolation or in combinations were abdominal pain, abdominal lump, ascites, chronic diarrhea, weight loss, fever and cough. Upper gastrointestinal endoscopy was done in 43 patients and suspected finding noted in 9 (21%) and Colonoscopy was done in 40 patients and ulcerations were found in 32 patients. Pan-endoscopy was done in 39 patients; endoscopic abnormality noted in 32 (82%) and diagnosis of luminal tuberculosis was made in 26 (81%) and AFB demonstrated in 11 (34%). %). All patients received standard anti-tuberculous treatment, with the exception of one death, all improved on follow up. Conclusion – Pan-endoscopy help in diagnosis of abdominal tuberculosis in HIV negative patients