Tumor markers are the substances, which quantitatively change in serum, during tumor development. Malignant cells have a distinctive type of metabolism with altered biochemical parameters which are either increased or decreased. One such marker is serum LDH. The aim of this study was to evaluate serum LDH along with some other biochemical parameters like alkaline phosphatase, SGOT, SGPT in histopathologically confirmed cases of OSCC during various clinicopathological stages and also postoperatively for prognostic implication. The study group constituted of 60 randomly selected subjects from the outpatient clinic who were histopathologically confirmed cases of oral squamous cell carcinoma. Serum LDH level may serve as an adjunct biochemical tool to provide collaborative evidence in diagnosis of OSCC.