Evaluate The Phenotypic And Genotypic Variations For Isolates Of Trichophyton Spp, Isolated From Different Habitats

Abstract

Identification of dermatophytes using conventional methods is unstable because macro- and micromorphological characteristics of these fungi are not clear. Therefore the aim of this study was to evaluate the phenotypic and genotypic variations using specific primer for dermatophytes and Trichophyton genus and sequencing of ITS region. By conventional morphological methods identified 105 isolates of Trichophyton spp. collected from different habitats (76 from human, 17 from animals and 12 from soil). Seventy four (74) isolates of Trichophyton spp. were used for molecular identification by using specific primer pair for dermatophytes had monomorphic bands of approximately PCR product of 366 bp and specific primer pair for Trichophyton genus had monomorphic bands of approximately PCR product of 258 bp. In addition to Trichophyton isolates were sequenced and analyzed by compared the sequence alignment with sequences from NCBI Blast database. These sequences are useful for identification of isolates have not been able to reach species level. Genotypic methods provides a very easy and accurate methods.