Molecular Taxonomy Of Galactomyces Spp. And Dipodascus Capitatus Associate With Dairy Based On Rdna Sequence Analysis In Iraq

Abstract

Galactomyces spp is a telomorph of Geotrichum candidum (anamorph name). is used as a culture for cheese making and in some traditional fermented milks, few studies have assessed the genetic diversity of Galactomyces spp that exist in traditional cheese making facilities. The aim of this study isolation and molecular taxonomically treated of Galactomyces spp and other Candida spp. correlated with dairy products. The results showed that most dairy samples companioned with two species of Galactomyces: G.candidum and G.geotrichum , D. capitatus and four species of Candida spp.: Candida krusei , C. kefyr, C. utilis and C.glabrata .A total of 23 fungal isolates were diagnosed based on universal primers ITS1 / ITS4 to amplify the Internal transcribed region spacer. Four doubtful G.candidum isolates showed unique sizes of products PCR ranged between 380-400 bp. other Candida PCR product ranged 420-780bp. Sequence analysis identified a doubtful G.candidum into G. candidum and G.geotrichum with 97% similarities and with D. capitatus showed 93% similarities, few intraspecific variation were observed at 312-380bp in the amplicons from the primer pair ITS1-ITS4 commonly are from 380 to 400 bp for G. candidum , G.geotrichum and D. capitatus and other variation in the ITS region of Candida spp under our interest .