Abstract

The taxonomy of the most Candida species especially C. parapsilosis and C. glabrata have undergone changes due to confuse in some taxonomic characters such as their color on CHROMagar and biochemical properties compare with accurate molecular method. The aim of this study was to evaluate the feasibility of using sequencing of the ribosomal internal transcribed spacer (ITS) region for identification of 18 Candida isolates. Preliminary CHROMagar assay and amplified ITS regions by primer pair ITS1/ITS4 and sequenced . Species identification performed based on the results of sequences which comparison by BLAST searches for homologous sequences in public databases. The CHROMagar assay results showed confuse in colonies colors of both species revealed white-pink color for both species .The results of PCR assay , rDNA sequences analysis and phylogenetic analyses showed accurate methods for identification of both species, Significant variation was observed among PCR products for 23 Candida species :511bp for C.parapsilosis 720 bp for C.kyfer and 780bp for C.glabrata .Sequence analysis show variation between C.glabrata, and C.parapsilosis and phylogenetic analyses produced different trees relative to analyses with consensus sequences. In conclusion, the CHROMagar candida medium unreliable test, showed high similarity in their color (white –pink color )characters for both C. parapsilosis and C. glabrata ,while the results of ITS sequencing provides a very accurate and useful method for the identification of Candida species in general trend , and C. parapsilosis and C. glabrata were showed distinct species based on sequences of ITS (rDNA).