Burns remain a major public health problem all over the world, especially in developing countries. Pseudomonas aeruginosa is a major cause of infection and a contributing factor in the death of patients with burns. So, there is a growing need to develop drugs which will prevent infections and complications more effectively than the presently used drugs. Ocimum sanctum L. (Holy basil in English and Tulsi in Hindi) has been shown to have antioxidant, antimicrobial, anti-inflammatory and wound healing properties. The present study was carried out to evaluate the epithelial regeneration property of Ocimum sanctum in thermal burns with and without supportive treatment of Ointment Silver Sulfadiazine. After the end of treatment at 28th day, group treated with Ointment Silver sulfadiazine + Ocimum sanctum showed significant epithelial generation (p-value 0.02, p<0.05 Significant).